纤维分解酶与异丁酸对犊牛小肠消化酶活力和肝生长轴基因表达的影响

doi:10.11843/j.issn.0366-6964.2016.09.017

摘要/Abstract

摘要：

为探讨日粮添加纤维分解酶与异丁酸对犊牛小肠消化酶活性和肝生长轴激素受体基因表达的影响，试验选取30日龄健康、体重近似的荷斯坦公犊36头，随机分为4组，对照组饲喂基础日粮，纤维分解酶组（FE）、异丁酸组（IB）和混合添加组（IBFE）在基础日粮上分别添加1.83 g纤维分解酶（FE，含160 U纤维分解酶和4 000 U木聚糖酶）、6.0 g异丁酸（IB，浓度98.5%）和两者混合物。分别在45和90日龄从每组中抽取3头屠宰并采集各肠段小肠内容物、肝样品，测定小肠消化酶活性和肝生长轴激素受体基因表达。结果表明，45日龄，IBFE组空肠近端、空肠远端和回肠的淀粉酶活性显著高于对照组和FE组（P<0.05）；IBFE组十二指肠、空肠近端、空肠远端和回肠胰蛋白酶活性显著高于其他处理组（P<0.05）；IBFE、IB组空肠近端和空肠远端脂肪酶活性均显著高于对照组（P<0.05）；IBFE和IB组GHR mRNA、IGF-ⅠR mRNA表达量显著高于FE和对照组(P＜0.05)，IBFE组IR mRNA表达量显著高于IB、FE和对照组(P＜0.05)。90日龄，IBFE组十二指肠和回肠的淀粉酶活性显著高于对照组和FE组（P<0.05）；IBFE组空肠近端、空肠远端和回肠胰蛋白酶显著高于其他处理组（P<0.05）；IBFE组十二指肠、空肠近端、空肠远端和回肠脂肪酶活性显著高于对照组和FE组（P<0.05）；IBFE、IB、FE组GHR mRNA表达量显著高于对照组(P＜0.05)，IBFE组IR mRNA表达量显著高于其他处理组(P<0.05)，IBFE和IB组IGF-ⅠR mRNA表达量显著高于FE和对照组(P＜0.05)。结果提示，日粮中添加纤维分解酶和异丁酸混合物对提高犊牛小肠消化酶活、肝生长轴激素受体基因表达效果最佳。

Abstract:

The objective of this study was to evaluate the effects of fibrolytic enzymes，isobutyrate or their mixture on digestive enzyme activity of small intestine and growth axis hormone receptor gene expression of liver in dairy calves.Thirty-six Holstein male calves of 30 days of age with similar weight were randomly divided into 4 groups.Calves in control group were fed basal ration.Calves in treatments were supplemented fibrolytic enzymes (FE，1.83 g per calf per day)，isobutyrate (IB，98.5% of isobutyrate，6 g per calf per day) and their mixtures (IBFE，FE + IB)，respectively.At 45 and 90 days of age,3 calves from each group were randomly selected and slaughtered.Samples of small intestine contents and liver were collected in the morning before feeding on 45 and 90 days of age for determining intestinal digestive enzyme activity，GHR，IGF-IR and IR mRNA expression in liver.The results showed that on 45 days of age，amylase activities of proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than FE and control groups(P＜0.05).Trypsin activity of duodenum digesta，proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than other groups(P＜0.05).Lipase activities of proximal jejunum digesta and distal jejunum digesta in IBFE and IB groups were significantly higher than control group(P＜0.05).The mRNA expression of GHR and IGF-IR in liver in IBFE and IB groups were significantly higher than that of FE and control groups(P＜0.05).The mRNA expression of IR in liver in IBFE group was significantly higher than other groups (P＜0.05).On 90 days of age，amylase activities of duodenum digesta and ileum digesta in IBFE group were significantly higher than that of FE and control groups(P＜0.05).Trypsin activities of proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than other groups(P＜0.05).Lipase activities of duodenum digesta，proximal jejunum digesta，distal jejunum digesta and ileum digesta in IBFE group were significantly higher than that of FE and control groups(P＜0.05).The mRNA expression of GHR in liver in IBFE，IB and FE groups were significantly higher than that of control group(P＜0.05).The mRNA expression of IR in liver in IBFE group was significantly higher than other groups(P＜0.05).The mRNA expression of IGF-ⅠR of liver in IBFE and IB groups were significantly higher than that of FE and control groups(P＜0.05).Intestinal digestive enzyme activity，mRNA expression of GHR，IGF-IR and IR in liver were improved by the supplementation of the mixture of fibrolytic enzymes and isobutyrate in calves.

中图分类号:

S823.5
S816

张莹莹，王聪，刘强，张延利，裴彩霞，王永新，郭刚，霍文婕，张拴林. 纤维分解酶与异丁酸对犊牛小肠消化酶活力和肝生长轴基因表达的影响[J]. 畜牧兽医学报, 2016, 47(9): 1879-1887.

ZHANG Ying-ying，WANG Cong，LIU Qiang，ZHANG Yan-li，PEI Cai-xia，WANG Yong-xin，GUO Gang，HUO Wen-jie，ZHNAG Shuan-lin. Effects of Fibrolytic Enzymes and Isobutyrate on Digestive Enzyme Activity of Small Intestine and Growth Axis Hormone Receptor Gene Expression of Liver in Dairy Calves[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1879-1887.

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